![]() ![]() ![]() My colleagues and I from SJSU’s Virtual Center for Archives and Records Administration (VCARA) and the Community Virtual Library are doing our 3rd annual live reading for this month’s Dickens’ Project. Now can you see why I’m so passionate about virtual worlds? But wait….there’s more! December Virtual Events in Second Life When I went in to get the above screenshot of my exhibit for this blog post, here’s a comment that was waiting for me: I created this exhibit because I think virtual community is now more important than ever, and coincidentally I have a great example to share of the kind of impact this kind of thing has on me and others. Vivian, the legendary Ruth Bader Ginsburg, and at the center altar there is an image of the COVID19 virus, representing the many people lost to us this year globally from the pandemic. Among the photos were George Floyd, Lorena Borjas (Mexican-American transgender and immigrant rights activist), civil rights icons John Lewis and C.T. I created several altars that represent not just one individual, but whole communities who have lost important people that represent them. When you land at the exhibit you are greeted by the serene sound of crickets, frogs, and the occasional hoot of an owl in an otherwise quiet small cemetery. On Halloween, I put up a public exhibit for the Second Life community a Día De Los Muertos scene honoring some of those we lost in 2020. Día De Los Muertos exhibit, in Second Life ![]()
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![]() ![]() ![]() We recommend running 20 μg of TSA-treated cell lysate as a control.ī) Carefully remove the gel from the pre-cast assembly and equilibrate the gel in transfer buffer with constant agitation 60-120 rpm for 15 min at room temperature.Ĭ) Prepare PVDF membrane by cutting to gel size and placing in 20 ml of 100% methanol.ĭ) Assemble the blotting sandwich with coarse plastic filter supports provided by the electroblotter manufacturer. Standard liquid based polyacrylamide gel transfer system from Biorad or GE Healthcare or other supplier (we have found most mini-gel blotting apparatus works well, but you should avoid rapid dry blotting equipment as these are difficult to optimize and have in general high backgrounds).Ī) Prepare samples in 1x SDS-loading buffer and run on a 4-20% mini-gel with molecular weight markers and control sample. Gel sized Whatman 3M filter paper (two pieces).PVDF membrane, 0.2 micrometer pore size, from EMD Millipore.Make the solution fresh in a clean 1L bottle as the glycine is easily polymerized if left for a few weeks in a re-used bottle which leaves broad bands on the gel/blot. Transfer buffer, One liter of 25 mM Tris Base (3.0 g/l), 195 mM glycine (14.4 g/l), and 15% (v/v) methanol, pH should be 8.30 after dissolving Tris base and glycine in 835 ml nanopure water.A great control sample would be a recombinant over-expressed protein from E.coli or higher eukaryote cell culture. This can be accomplished by the second recommendation, 2) a simple equilibrating step of the gel in transfer buffer for 10 min with agitation, prior to assembling the transfer setup. Two general preliminary guidelines are useful for setting the foundation for successful western blots these are 1) SDS must be removed from the separating gel prior to transfer of proteins that are less than 100 kDal, failing to do this will allow the protein to pass straight through the membrane because its charge has been modified to a higher pI than the pH of the buffer by the sulphate groups of SDS. The standard conditions in the western blot protocol described below will be sufficient for efficient transfer and detection by quality antibodies. ![]() Over 70% of proteins fall into a typical category when considering western blot protocols. MemGlow™ Plasma Membrane Probes for Bioimaging 11.Small G-protein Activators & Inhibitors 5.GO-Blot - Fully Automated And Programmable Western Blot Processor.New Signal-Seeker™: PTM detection kits, antibodies, and reagents. ![]() ![]() ![]() This sheet helps you to track long-term expenses that you want to save for. Now, for savings, let’s start with our savings goal worksheet. Next, use the donations log to keep track of any donations you make throughout the year so that you can turn those in at tax time, as well. I don’t usually know the actual amount until a month or so before they are due, but I can estimate and have the majority saved before the time comes. One that always sneaks up on me is car tags and taxes! Unlike the car payment that comes each month, we pay these once per year, and if I don’t plan for it, it ends up being a big unexpected expense. It makes you able to space out saving up for bigger expenses. One of the best things you can do for your budget is to plan ahead and get prepared. Use the yearly expenses page to plan for variable expenses that might sneak up on you- saving for Christmas or birthdays is a great example of this. It can be important to track these, even if you don’t necessarily have a lot of health issues, because it helps when it comes time to deal with insurance, or at tax time! This was so helpful for me when my son was younger and we had a lot of different expenses going on. The next page I included was a medical expenses tracker. ![]() Or, get in the habit of paying certain things a week or two ahead so that they don’t pile up all at once. HINT: If you do find that you have a lot of bills stacked on one end of the month, you can call your creditors, or the companies you pay to, to see if it is possible to change your due dates. This helps you to space out when things are to be paid so that you don’t have most of your bills due at the beginning of the month, or so that if you do, you can plan for it! Knowing when you want to pay each bill is an important part of the budgeting process. The finance calendar is a great way to have a monthly overview of when you plan to pay your bills, so you know what to expect each week. ![]() I know that we have a lot of different due dates, and it can be hard to keep track, but this sheet makes it easy. This can help you save money by avoiding late fees or other penalties for paying late. The monthly bill checklist will help you keep track of your monthly bills that are paid and make sure nothing slips through the cracks. There are lots of lines if you need them, and you can also print more than one sheet if you find that necessary to track all of your spending. Use a new sheet each month and track everything you spend to know exactly where your money has gone. Next, the monthly expense tracker, or expenses log, is a sheet just for tracking your expenses on a monthly basis. ![]() Use this free budget worksheet to plan your personal budget every month. It has space for you to track your total income, your bills due, monthly expenses, and savings and debt goals. This is your main budget tracker sheet that is included and it provides a great budget overview. You can choose which sheet you prefer, depending on your paycheck frequency. Let’s take a closer look at what is included in this set- Free Budget Printables-įirst, is the weekly/biweekly or monthly budget planner page. Sometimes with goals, we start off with grand intentions only to stop working towards them because we don’t see much initial progress.These printables are only for personal use, but I do have a few different colors you can choose from! Also, they are made in both letter and half letter size, which can fit into A5 size planner binders! They are free for subscribers so scroll down and sign up to download these, and all of my free printables. Whether you’re saving up to have a rainy day or emergency fund, to go on a nice vacation, to put a downpayment on a house, to buy a car, or even to save up for big holidays like Christmas or birthdays, we have got 27 Free Printable Savings Trackers to help you achieve your goals! We all have something we’re saving for or would like to save for. ![]() ![]() ![]() This series of small games came out in 1980 and there was a Mickey Mouse version released shortly after in 1981, marking Disney and Mickey's first entry into the world of video games. Some of you old timers out there might remember that Nintendo started out making small handhelds known as Game and Watches. Disney and video games go back a long way, pretty much to the beginning. If you were to look closely in that vast collection of games spread out across my basement floor, you'd find an awful lot of Disney games. So, yeah, I guess I am a video game collector after all. In my house right now I still have that original, still functioning Atari from my childhood as well as my Nintendo Entertainment System, SEGA Genesis, Super Nintendo, original Playstation, Nintendo GameCube, SEGA Dreamcast, Nintendo Wii and my son's Nintendo Wii U, Playstation 4 and Nintendo Switch as well as just about every iteration of Nintendo's GameBoy and DS handhelds. So, me and video games, we go back a ways. Two things were under every tree that following Christmas of '77, Star Wars toys and and a brand new Atari game system. In 1977, at the perfect age of 9 we got Star Wars in May and then in September, we got the release of the Atari 2600. I never really considered myself a video game collector until that "Holy Cow" moment when i walked down the stairs and saw just how many games we have in the house. I keep my collections either out on shelves or neatly stored away and intact. Thankfully, I'm far away from being featured on an episode of Hoarders. Me? As many of you readers know, I'm a collector, an accumulator. ![]() When I sent the photo to my daughter, her reply was, "That's crazy! You have so many…get rid of them! You can't possibly play all of them anymore." That's the practical side of the family speaking for sure. The games stayed that way for several weeks as my sons and I tried to play through some of them that have been left alone for quite some time. One of my sons decided to take out every single video game we had from all of our systems acquired over the years and lay them out on the floor. I couldn't get all of the games in one shot. This is only a portion of my basement floor. For instance, one day I walked down the stairs to the basement rec room and found this: Still, we found plenty of interesting things to keep us busy. But you can't bake bread without yeast and, as some of you may have experienced, there was no yeast to be found back then. When I send an email out at work to my friends and colleagues that I've brought in some of Diane's baked treats a line forms out my door. We never dove into the whole baking bread thing in this house for example. For most of us, the early months at home brought with them some interesting developments. It goes without saying that all sorts of strange things have been happening over the last 6 months of this pandemic. As always, I hope you are all safe and healthy out there. ![]() ![]() ![]() But what makes it different from most other maps is that you’re going to have to purchase an entire farm to get access to the fields. Autumn Oaks contains 4 individual farms and only 14 fields to play around with. That’s the only way to stay ahead of everything. If you’re looking for a completely unique FS17 experience, Autumn Oaks might be a good choice. ![]() Take the best that you can and don’t look back – you must be orientated to the future and new experiences. This is an American styled map based around small family farms. That could be a great boost to your farm and there are no reasons why to react negatively. It’s great getting even more than it was expected – that’s why we insist on you to try and test the benefits personally. It’s amazing how much a simple update can do – be the smart player and achieve much more than you were ever planning. There is no risk, so no arguments against – therefore the are no excuses of not taking this special chance. Download Farming Simulator 2017 mods now and dive into the most exciting adventures of your life DOWNLOAD Trending FS17 Maps mods Zillertal Alps V 2.0 FS17 Los Grandes Terrenos 2017 V 1.0.9. These additional files will add so much value that you won’t want to play without them anymore. This few simple steps will lift your game to the upper level – what can be better that upgrading your game personally? Break all boundaries and develop your farm easily. Then all you need to do is simply click on LS19 Mods / LS22 Mods free download button and implement it to your game. Take a deeper look at our offered samples and pick the needed one. If you are passionate about the Farming Simulator 19 Mods / Farming Simulator 22 Mods as we are, then you are on the right site. If you wonder how it works, you will find plenty of information in our FS19 Mods / FS22 Mods website – we have collected all kind of data to make the gaming easier. ![]() FS17 Autumn Oaks (3) (No Ratings Yet) Loading. That’s definitely for a reason – every single Farming Simulator 19 Mod / Farming Simulator 22 Mod is unique and provides you explicit options. FS Mods on Consoles FS System Requirements FS Release Date FS Facebook Giants Editor Help Contacts FS17 Autumn Oaks (3) FS15 Mods FS17 Mods FS19 Mods FS22 Mods 18 Dec, 2017 17:04. There additional files are extremely popular all around the globe. If you are face difficulties which are hard to overcome, check our Farming Simulator Mods database and you will probably find a solution there. You have successfully joined our subscriber list. ![]() ![]() ![]() The more there are, the more you’ll need your small poster design to make a lasting impression. There are a lot of posters up there competing with each other, and most are going to be printed in the smaller poster size dimensions. Think of bulletin boards, community boards, and the like. Small posters are great for getting your message across without taking up too much room. Small Poster Size: 11” x 17” (28 x 43 cm) The busier it is, the harder it will be to read, thus diminishing its impact. Since they are smaller, you do want to be careful about how much text you are putting on it. These poster dimensions are also referred to as Architectural C Size. If you’re looking to create a smaller advertisement, or if your design leans more towards the minimalist side, then consider using a medium poster size. Medium Poster Size: 18” x 24” (46 x 61 cm) If you want to create your own cool poster, then this is the size you should look at using. The large poster dimensions are also what most people use for decorating their rooms or posting up at local businesses for promoting their cause, or club, for instance. ![]() Large posters aren’t only used by professional companies. This size can also be referred to as Architectural D Size. When you walk past a poster in the mall, at a festival, or a tradeshow, then this is typically the size that is used for those. Large Poster Size: 24” x 36” (61 x 91 cm) Here’s a handy list of standard poster dimensions for you to familiarize yourself with. What it comes down to is that there will always be slight variations in sizes, especially from country to country, but there are ballpark dimensions that each size falls into internationally. The Way, Way Back is a lovely color, but the title is illegible unless blown up much larger.There are no official standard poster sizes, but there are certain design dimensions that the printing industry abides by. In the above selection of posters for current films, the best of the bunch is clearly The Heat - the red color stands out, the film title is obvious even at that size and the clean graphics look fine when shrunk down. ![]() You also want a basic JPG that looks clear when shrunk to about 2″ x 1″
![]() ![]() Admissions Straight Talk Podcast for College Applicants.Admissions Straight Talk Podcast for Healthcare Applicants.Once that’s finished, the last step is proofreading, where you fix all the grammar and spelling mistakes and give it a final lookover. The editing phase is when you finally take care of all the issues you let slide while writing the rough draft. Just like you learned how to write a rough draft, you should follow the best practices for how to edit as well. Follow your outline as best you can, but also keep an open mind for new ideas-writing the first draft can be full of inspiration! After the rough draft: Next stepsĪfter the first draft, you’re ready to revise. Instead of nitpicking, just focus on solidifying your raw ideas. Finessing the technical, finer points of writing like word choice and grammar are easier after the first draft, so compartmentalize and save them for later. This is because if you’re focusing on finding the right word or making sure your grammar is correct, it means you’re not focusing on the big picture. As we’ve said, the goal of a rough draft is to get all your ideas down, not to write everything perfectly on your first try. The most important tip for writing rough drafts is to give yourself permission to write imperfectly. With those out of the way, you can begin your first draft with confidence. You can think of your outline as the rough draft for your rough draft-a blueprint of where everything goes. You’ll come up with new ideas while writing the rough draft, but it’s always best to stockpile as many as you can beforehand.Īfter brainstorming comes the outlining phase, which is essential for structuring your content and putting everything in a logical order. Beginning with the brainstorming process is crucial for eliciting and organizing all the content you want to put in your writing, whether it’s creative ideas for fiction or supporting evidence for nonfiction. How to write a rough draftįor starters, your first draft is not the first step. It’s useful to have all the material you need for your final draft already in your first draft, including citations, so you can gauge the piece as a whole. Throughout the editing process, keep in mind that what you ultimately keep should contain the best parts of that rough draft. This can actually work in your favor, giving you more usable material to choose from. Many writers tend to overwrite their first drafts. ![]() Specifically, let’s answer two frequently asked questions about what to include in a rough draft: How long should a rough draft be?Ī rough draft should be about as long as the final version. Of course, the rough draft is only for the writer, so no one will stop you if you need to skip some sections or gloss over others-but you’ll have to address any shortcuts leading up to the final draft. ![]() So, everything you’d include in the final draft should go into the first draft. ![]() What to include in a rough draft?Ī rough draft is essentially a raw version of the complete assignment. Certain flaws like organizational issues or plot holes aren’t evident until after you see them laid out in writing. Finding the perfect word and arranging elements in the best order are much easier after you have a first draft, whereas they can be quite troublesome and time-consuming without one.Ī rough draft also helps you identify problematic areas you can’t see with outlining and brainstorming alone. The ultimate goals of your rough draft are to get your ideas down and give yourself something to start with. Remind yourself: It doesn’t have to be good, it just has to be. When embraced with the right mindset, the rough draft helps cut through these obstacles by taking the pressure off. Even if you have a talent for putting words together, you’re not immune to the concerns that beset any writer, such as deadline dread, creative blocks, or any number of personal insecurities. Often, it’s the longest and most effortful phase, involving most of the actual “writing.” What’s the purpose of a rough draft? The rough draft is part of the recommended writing process, step three of five to be exact. The idea is to get a large portion of your project actually written, and worry about fixing the problems later. A rough draft is never intended to be perfect it’s full of grammatical mistakes, poor word choices, and structural issues. It acts as the framework for the final version. Grammarly helps you communicate confidently Write with Grammarly What is a rough draft?Ī rough draft or first draft (or, according to my second-grade teacher, a “sloppy copy”) is an initial, incomplete piece of writing that is the first attempt at getting all your ideas on paper. ![]() ![]() The content created by our editorial team is therefore objective, factual and not influenced by our advertisers. Our editors, journalists and reporters create independent and accurate content to help you make the right decisions. WEBSEITENNAME follows a strict editorial policy, so you can trust that our content is honest and independent. We constantly strive to provide our readers and consumers with the expert advice and tools they need to succeed throughout their life journey. Our experts, journalists and editors have been helping our readers with everyday questions and decisions for over many years. You are wondering how we make money and stay independent. Our primary goal is to provide you, our reader, with added value and to assist you with your everyday questions and purchasing decisions. Our editors and experts have years of experience in researching and writing reader-oriented content. How can we earn money and stay independent, you ask? We'll show you. You can therefore rely on the independence of our editorial team. Our authors do not receive any direct remuneration from the advertisers on our pages. To ensure that our editorial standards are not influenced by advertisers, we have established clear rules. We want to help you solve everyday problems and make the right decisions. The best advice for you - that is our greatest goal. You as a reader are the focus of our editorial work. Therefore, our specialist editorial team does not receive any direct remuneration from advertisers on our pages. We draw a clear line between our advertisers and editorial staff. Therefore, we have set editorial standards based on our experience to ensure our desired quality.Įditorial content is vetted by our journalists and editors to ensure our independence. We want to provide our readers with objective information that keeps them fully informed. The authors of WEBSEITENNAME research independent content to help you with everyday problems and make purchasing decisions easier. Therefore, you can be sure that your interests are our top priority. Please DIY finding and using a suitable blocking list for your chosen BT protocol application.WEBSEITENNAME operates according to an established editorial policy. Be extra careful out there! And again, an up-to-date blocking list is recommended. You can read about BIglyBT here at MU as well as at the URL provided below by Barry5p. Head over to Wikipedia for excellent introductions to all these topics and applications, with the exception of BiglyBT, which apparently hasn't yet hit Wikipedia's radar. As such, never use WAREZ, or at least first scan such software with a high quality and up-to-date anti-malware app before using it. Also note that BitTorrented WAREZ software is now infamous for being infected with malware. Keep in mind that BT sharing remains under scrutiny by the media industry. The BitTorrent protocol has a steep initial learning curve, but is well worth the effort. As such, it provides considerably more options than Transmission. It is considered the descendent and revival of the Azureus/Vuze project. The BiglyBT Open Source project began in 2017 in response to the Vuze situation. It is considered the most simple to setup and use reliably. In 2005 the Transmission Open Source project was started and continues on as such today. In 2010, Vuze was purchased by Spigot, became proprietary, was parallel named µTorrent, is now considered ADWARE and should be avoided. Meanwhile, in 2004 the Open Source Azureus project was started and continued on via Vuze. BitTorrent is currently considered ADWARE and should be avoided. The cryptocurrency TRON Foundation bought the BitTorrent company in 2018, at which point creator Bram Cohen left the company. As of version 6, BitTorrent became proprietary software. As a result, BitTorrent was forced to 'play nice' with the media industry or face lawsuits. As of version 5 in 2006, BitTorrent came under the scrutiny of the MPAA (Motion Picture Association of America) and the RIAA (Recording Industry Association of America) due to copyright infringement by BitTorrent users. Azureus and its descendents are based on this code. Versions 1 - 4 of their software were Open Source via various licenses. The BitTorrent company was formed in 2004. ![]() It is also the name of the original, very first BT client, created by Bram Cohen in 2001. "BitTorrent" (BT) is the name of a file sharing protocol. If you like bells and whistles, aka Geek Meter in the RED, you want BiglyBT. I personally use the latest version of Transmission along with an up-to-date blocking list. ![]() NO, this is not the torrenting client you're looking for. ![]() ![]() ![]() At this step, just select this folder on your computer. We suggest creating an empty folder beforehand. Installed Photoshop-compatible 64-bit plugins can be made available to Affinity Photo to extend the range of effects available to you. During the “Compatible Host” step, click on + to create a custom plugins folder in your preferred directory (use the combination shift+Cmd+n if necessary), then click Open.To log in to your Customer Account directly from this article, please click on this link. It offers 4 directions of isometric conversion, a flexible cuboid generator and a 50px isometric grid. To install Nik Collection’s latest version you have licensed, the download can always be found in your customer account. This is a unique Photoshop plugin suitable for creating realistic isometric objects that you can use when creating websites and developing games. To do this go to menu Edit Preferences, Photoshop Plugins section, click the Add button and navigate to the Photoshop plugins folder then click Select Folder on the bottom right of the dialog. To see the compatibility tables with operating systems and third-party software directly from this article, please click on this link. Please check the compatibility chart before installation, and make sure you have installed either the latest version of Nik Collection 4 or any version of Nik Collection 5, and Affinity Photo (1.10.3 or later) if you are using Apple M1 processors. ![]() To view the user guide directly from this article, please click on this link, and select the language of the software concerned. Using Affinity Photo and the Nik Collection together requires a special installation procedure which is described in the user manual. This information applies to version(s): 4, 5 Once enabled, open the plugin from the Filters menu. This information applies to version(s): 6įor users of Nik Collection 6, the plugins will be automatically installed in the correct folder of compatible versions of Affinity Photo. The Photo AI plugin for Affinity Photo is not automatically enabled but can be manually added. ![]() ![]() ![]() I don’t like the deficit it creates at running back or receiver, and I see many other paths to landing a possible top-five quarterback. Ascending talents.Įarly in the draft cycle, I thought I might be open to chasing the top tier of quarterbacks, where Patrick Mahomes, Josh Allen, and Jalen Hurts will go. You want as many players as possible who are still on the escalator, who haven’t had their best season yet. The longer I play fantasy football, the more I understand the importance of having a young team. Sometimes it’s no fun playing fantasy football like an actuary, but it’s a prudent thing to do. If the discount isn’t significant on draft day, I’ll avoid those already likely to miss time. Injuries will find you, don’t go looking for them. I’m not a manager who drafts into injured players. There are more right answers as we are content with less production. The bell cow is almost dead in the NFL, and although you might think that makes running back picks more stressful, it actually has the opposite effect. You’ll probably be pleasantly surprised at how sturdy the RB2/RB3 tier is, in part because we’re not expecting those slots to carry as much weight as they did in prior seasons. In those leagues that are more receiver driven, I probably will look to have one anchor running back, and then I’ll circle back to that position later. If a league requires just two starting wideouts, then my early running-back build will be equal to my wide-receiver build. If a league requires at least three wide receivers, I want to have the best wideout room in the league, or pretty close to it. I play fantasy football with a microscope, not a telescope. I’ll start looking to the money weeks in the middle of the season, but my goal right now is to be good in the short-term, to win September. I’ve generally thought planning for Week 17 before the season is somewhat of a fool’s errand. There are some common mistakes that fantasy managers, even good ones, often make. And any strategy can lose if you pick the wrong players. It’s all contextual.Īny strategy can win if you pick the right players. General strategy and adviceĪny tips are to be taken with a grain of salt. Your fall happiness depends on what happens in the next 72 hours or so. This is it, the biggest fantasy football draft weekend of the year. ![]() |
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